Generator

Part:BBa_K1021034:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-15)


PtrpC+hph+TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1894
    Illegal AgeI site found at 714
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1610


Design Notes

This part was designed as an improvement to BBa_K1021030.


Source

The promoter and terminator originating from Aspergillus nidulans, as well as the hph gene from E. coli, were taken from the pUCATPH transformation vector.

References